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The cells were lysed in RIPA buffer (MilliporeSigma) supplemented with protease and phosphatase inhibitors (BioTools)

In laboratory models, DSIP is associated with modulation of the hypothalamicpituitaryadrenal axis and broader neurochemical signaling processes that govern circadian rhythm, hormonal release, and cellular stress adaptation

Douros A, Filion KB, Yin H, et al